Description
Perform large-scale isolation of high quality plasmid DNA from recombinant
E.coli cultures with Thermo Scientific™ Fermentas GeneJET™ Plasmid Midiprep Kit.
The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription and transfection of robust cell lines.
Highlights - Efficient – High yields up to 200 µg of high quality plasmid DNA from 50mL of bacterial culture
- High concentration of DNA purified – 400 ng/µL
- Convenient – Spin column format, centrifuge or vacuum protocols
- Fast – Procedure takes 60min, considerably faster than using gravity flow columns
Includes: Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10 mM Tris-HCl, pH 8.5), GeneJET™ Midi Purification Columns pre-assembled with collection tubes (15 mL), Collection tubes (15 mL)
Recommended for: FastDigest™ restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,
in vitro transcription, transfection of robust cell lines